Role of protein in the primary step of the photoreaction of yellow protein.

Cloning and Expression of the Coat Protein Gene of Barley Yellow Dwarf Virus-PAV in Escherichia coli

Molecular detection of proteolytic activity of human parechovirus 2A protein by gene expression

  Parechoviruses form one of the nine genera in the picornaviridae family, and include two human pathogens: Human parechovirus type1 and 2 (Hpev1 and Hpev2). The genome of picornaviruses encodes a single polyprotein, which undergoes a cleavage cascade performed by virus encoded proteases to give the final virus proteins. The primary cleavage occurs by 2A protein and this step is critical for vi...

A New Two Step Induction Protocol for Neural Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells

Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incu...

همسانه‌سازی و بیان ایمونوتوکسین اونتاک به صورت هیبریدی با دنباله اینتئینی

Introduction: Inteins (INT) are internal parts of a number of proteins in yeast and some other unicellular eukaryotes, which can be separated from the immature protein during protein splicing process. After identifying the mechanism of intein action, applications of these sequences are be considered in the single- step purification of recombinant proteins and different intein tags were develope...

Acute and chronic effects of lithium on BDNF and GDNF mRNA and protein levels in rat primary neuronal, astroglial and neuroastroglia cultures

Objective(s):Theneuroprotective effect of lithium has been attributed to its therapeutic action. However, the role of glial cells particularly astrocytes, and the possible interactions between neurons and astrocytes in neuroprotective effects of lithium have been disregarded. Thus, the aim of this study was to evaluate the direct effects of lithium on brain derived neurotrophic factor (BDNF) an...